Comparison of Immune Effects Between Brucella Recombinant Omp10-Omp28-L7/L12 Proteins Expressed in Eukaryotic and Prokaryotic Systems
Lin Zhu, Qiuju Wang, Yujian Wang, Yulin Xu, Duo Peng, He Huang, Liping Hu, Kai Wei, Rui‐Liang Zhu
Abstract
Brucella Subunit Vaccine of TPPPS adjuvant could significantly improve the protective effects of subunit vaccines. However, we also noticed that all of the evaluated subunit vaccines induced less protection than the B. melitensis M5 live vaccine. These results indicate that the combination of recombinant Omp10-Omp28-L7/L12 antigen and TPPPS adjuvant shows potential as an effective brucellosis subunit vaccine, and P. pastoris is a preferred expression system to prepare this recombinant subunit antigen.
Topics & Concepts
BrucellaBiologyImmune systemRecombinant DNAImmunogenicityMicrobiologyVirologyPichia pastorisAttenuated vaccineAdjuvantImmunologyBrucellosisVirulenceGeneBiochemistryBrucella: diagnosis, epidemiology, treatmentMonoclonal and Polyclonal Antibodies ResearchGalectins and Cancer Biology