A label-free multiplex electrochemical biosensor for the detection of three breast cancer biomarker proteins employing dye/metal ion-loaded and antibody-conjugated polyethyleneimine-gold nanoparticles
Kulrisa Kuntamung, Jaroon Jakmunee, Kontad Ounnunkad
Abstract
, respectively, which are significantly lower than the clinically relevant cut-off levels. The sensor reveals high selectivity and satisfactory reproducibility. After storing for two weeks, the sensor retains the responses with ca. 90% of the initial currents. The immunosensor is successfully applied to detect three tumor markers in human serum and can provide a new technological platform for the development of low-cost, highly stable, sensitive, selective, and point-of-care (POC) diagnosis.
Topics & Concepts
MultiplexConjugated systemColloidal goldBiosensorBreast cancerMetal ions in aqueous solutionElectrochemistryMaterials scienceBiomarkerNanotechnologyImmunoassayAntibodyNanoparticleChromatographyChemistryCombinatorial chemistryMetalCancerBiochemistryElectrodeMedicineBiologyPolymerImmunologyBioinformaticsComposite materialMetallurgyPhysical chemistryInternal medicineAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionElectrochemical Analysis and Applications