Development of magnetic nanoparticles for the intracellular delivery of miR-148b in non-small cell lung cancer
Julien H. Arrizabalaga, Jonathan S. Casey, Jeffrey C. Becca, Yiming Liu, Lasse Jensen, Daniel J. Hayes
Abstract
In this study, we describe the development of an alternating magnetic field radiofrequency (AMF-RF) mediated siRNA delivery platform for the intracellular release of miRNA mimic in A549 cells. Iron oxide nanoparticles were synthesized with discrete chemical attachment sites for the conjugation of payloads. The delivery of the miRNA mimic was controlled by a thermally labile Diels-Alder linker, tethering the oligonucleotide to the surface in an inactive state. The Diels Alder linker acted as an effective control switch to spatiotemporally deliver the therapeutic payload via AMF-RF stimulation. Using confocal microscopy imaging, we demonstrated nanoparticle uptake and controlled intracellular release of the miR-148b mimic from the nanoparticles when stimulated with AMF-RF. Additionally, cell viability and proliferation assays indicated a significant reduction in cancer cells compared to control groups.