AGO-accessible anticancer siRNAs designed with synergistic miRNA-like activity
Dowoon Gu, Seung Hyun Ahn, Sangkyeong Eom, Hye-Sook Lee, Juyoung Ham, Dong Ha Lee, You Kyung Cho, Yongjun Koh, Elizaveta Ignatova, Eun-Sook Jang, Sung Wook
Abstract
xenograft models (206/E7). Other mi/siRNA sequences were additionally designed for cervical, ovarian, and breast cancer, and available as an online tool (http://ago.korea.ac.kr/misiRNA); some of the mi/siRNAs were validated for their augmented anticancer activity (206/EphA2 and 206/Her2). mi/siRNAs could coordinate miRNA-like activity with robust siRNA function, demonstrating the potential of AGO CLIP analysis for RNAi therapeutics.
Topics & Concepts
Small interfering RNARNA interferencemicroRNATrans-acting siRNABiologyGene silencingArgonauteRNAComputational biologyGeneGeneticsMicroRNA in disease regulationRNA Interference and Gene DeliveryRNA Research and Splicing