Expression of <b><i>AdipoR1</i></b> and <b><i>AdipoR2</i></b> and Serum Level of Adiponectin in Gastric Cancer
Morteza Kordafshari, Mahyar Nourian, Narjes Mehrvar, Hassan Jalaeikhoo, Aida Etemadi, Alireza Khoshdel, Mohammad Ghaznavi Idris, Shahrokh Iravani, Azim Mehrvar
Abstract
<b><i>Background:</i></b> Cancer is one of the major causes of death worldwide and the third leading cause of death in Iran. One of the proteins that are considered having anticancer effects is the adiponectin hormone. Adiponectin leads to programmed cell death, prevents cell growth and proliferation, and increases the expression levels of <i>BCL2</i>. <b><i>Aim:</i></b> The aim of this study was to assay the expression of adiponectin receptors (<i>AdipoR1</i> and <i>AdipoR2</i>) genes in gastric cancer patients. <b><i>Materials and Methods:</i></b> In this case-control study, 42 gastric cancer patients and 52 volunteers as healthy controls were enrolled. Total RNA was extracted. cDNA was synthesized by the reverse transcription method, and expression analysis was performed by real-time PCR. The serum level of adiponectin was also measured by ELISA. <b><i>Results:</i></b> The expression of both <i>AdipoR1</i> and <i>AdipoR2</i> was significantly higher than the control group (<i>p</i> = 0.02). Serum adiponectin was significantly lower in gastric cancer cases when compared with normal controls (<i>p</i> = 0.03). <b><i>Conclusion:</i></b> We found that expression level of <i>AdipoR1</i> and <i>AdipoR2</i> is strongly higher; however, the level of circulating adiponectin is lower in gastric cancer. Our study suggests that the expression of <i>AdipoR1</i> and <i>AdipoR2</i>, besides the low level of adiponectin, may play an important role in the development and/or progression of gastric cancer.