Engineering the oleaginous yeast <i>Yarrowia lipolytica</i> to produce nutraceuticals: From metabolic design to industrial applications
Yang Gu, Xueyao Lu, Tongkang Liu, Yanjin Song, Ellie Sang, Shuran Ding, Long Liu, Feng Xue, Peng Xu
Abstract
Abstract The oleaginous yeast, Yarrowia lipolytica , has garnered much attention and is gaining recognition as a potential industrial platform for nutraceutical production. The superior properties of Y. lipolytica include its transparent inherited background, broad substrate spectrum, and superior environmental tolerance. Specifically, advances in genetic tools and engineering strategies have further widened its applications in metabolic engineering and biomanufacturing. In this review, we summarized developments in synthetic biology and engineering strategies for constructing Y. lipolytica to produce nutraceuticals. We also proposed perspectives on the limitations and challenges in developing microbial Y. lipolytica factories.