Bioorthogonal mRNA labeling at the poly(A) tail for imaging localization and dynamics in live zebrafish embryos
Kim Joana Westerich, Karthik Subramanian Chandrasekaran, Theresa Groß-Thebing, Nadine A. Kueck, Erez Raz, Andrea Rentmeister
Abstract
Live imaging of mRNA in cells and organisms is important for understanding the dynamic aspects underlying its function.
Topics & Concepts
ZebrafishLive cell imagingBioorthogonal chemistryMessenger RNADynamics (music)Cell biologyEmbryoFunction (biology)BiologyComputational biologyChemistryGeneticsPhysicsCellGeneClick chemistryCombinatorial chemistryAcousticsRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesRNA modifications and cancer