TRPA1-dependent calcium transients and CGRP release in DRG neurons require extracellular calcium
Lisa A. Gebhardt, Tatjana I. Kichko, Michael J. M. Fischer, Peter W. Reeh
Abstract
Shang et al. (2016. J. Cell Biol.https://doi.org/10.1083/jcb.201603081) reported that activation of lysosomal TRPA1 channels led to intracellular calcium transients and CGRP release from DRG neurons. We argue that both findings are more likely due to influx of insufficiently buffered extracellular calcium rather than lysosomal release.
Topics & Concepts
ExtracellularCalciumCalcitonin gene-related peptideCalcium in biologyChemistryIntracellularT-type calcium channelCell biologyBiophysicsVoltage-dependent calcium channelBiochemistryBiologyReceptorNeuropeptideOrganic chemistryIon Channels and ReceptorsNeurobiology and Insect Physiology ResearchPlant and Biological Electrophysiology Studies