Oleanolic acid: A promising antidiabetic metabolite detected in Aglianico grape pomace
Francesco Errichiello, Maria D'Amato, Angelita Gambuti, Luigi Moio, Arianna Pastore, Hekmat B. Al-Hmadi, Mariano Stornaiuolo, Elena Serino, Orazio Taglialatela‐Scafati, Martino Forino
Abstract
Grape pomace, a bulky component of winery waste, is a source of healthy compounds. So far, scientific research has mainly focused on its polyphenol content, but given the impressive number of bioactivities shown by grape pomace, it is not unlikely that, besides polyphenols, additional metabolites, so far undetected, may be involved. In order to verify such hypothesis, an in-depth chemical analysis of Aglianico (Vitis vinifera) grape pomace was conducted by NMR and LC-MS/MS. In addition to a number of polyphenols, a remarkable concentration of oleanolic acid (0.45 mg/g - fresh weight) was determined in the analyzed material. Oleanolic acid is a natural triterpenoid showing many bioactivities including antitumor, anti-inflammatory, antibiotic and antiviral properties. Also, it was proven a potential antidiabetic molecule in Type1 Diabetes rats. Hence, its influence on the mitochondrial and glucose uptake activities of C2C12 myoblast was here assessed, thus supporting oleanolic acid as a promising antidiabetic metabolite.