A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
Yun Shan Goh, Lisa F. P. Ng, Laurent Rénia
Abstract
One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021).
Topics & Concepts
SerologyFlow cytometryAntibodyCoronavirus disease 2019 (COVID-19)VirologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Spike ProteinCoronavirusImmunologyBiologyMedicineInfectious disease (medical specialty)DiseasePathologySARS-CoV-2 and COVID-19 ResearchSARS-CoV-2 detection and testingBiosensors and Analytical Detection