Elucidating Essential Genes in Plant-Associated Pseudomonas protegens Pf-5 Using Transposon Insertion Sequencing
Belinda K. Fabian, Christie Foster, Amy J. Asher, Liam D. H. Elbourne, Amy K. Cain, Karl A. Hassan, Sasha G. Tetu, Ian T. Paulsen
Abstract
Pf-5 genes and gain insights into gene functions and interactions that are not readily available using traditional methods. Comparing Pf-5 essential genes with those of three other pseudomonads highlights how gene essentiality varies between closely related species.
Topics & Concepts
BiologyTransposon mutagenesisGeneTransposable elementGeneticsGenomeMutagenesisIn silicoComputational biologyMutantPlant-Microbe Interactions and ImmunityGenomics and Phylogenetic StudiesPlant Pathogenic Bacteria Studies