Simultaneous screening of multiple substrates with an unspecific peroxygenase enabled modified alkane and alkene oxyfunctionalisations
Anja Knorrscheidt, Jordi Soler Soler, Nicole Hünecke, Pascal Püllmann, Marc Garcia‐Borràs, Martin J. Weissenborn
Abstract
Protein engineering of an unspecific peroxygenase (UPO) was performed with three substrates and six products in parallel by a high throughput GC-MS setup. Modified chemo- and regioselective variants were identified for aliphatic substrates.
Topics & Concepts
AlkeneAlkaneRegioselectivityChemistryCatalysisCombinatorial chemistryOrganic chemistryEnzyme Catalysis and ImmobilizationClick Chemistry and ApplicationsEnzyme-mediated dye degradation