Aerobic methane synthesis and dynamics in a river water environment
Abdullah M. Alowaifeer, Qian Wang, Brian Bothner, Ryan J. Sibert, Samantha B. Joye, Timothy R. McDermott
Abstract
Abstract Reports of aerobic biogenic methane (CH 4 ) have generated new views about CH 4 sources in nature. We examine this phenomenon in the free‐flowing Yellowstone river wherein CH 4 concentrations were tracked as a function of environmental conditions, phototrophic microorganisms (using chlorophyll a , Chl a , as proxy), as well as targeted methylated amines known to be associated with this process. CH 4 was positively correlated with temperature and Chl a , although diurnal measurements showed CH 4 concentrations were greatest during the night and lowest during maximal solar irradiation. CH 4 efflux from the river surface was greater in quiescent edge waters (71–94 μ mol m −2 d) than from open flowing current (~ 57 μ mol m −2 d). Attempts to increase flux by disturbing the benthic environment in the quiescent water directly below (~ 1.0 m deep) or at varying distances (0–5 m) upstream of the flux chamber failed to increase surface flux. Glycine betaine (GB), dimethylamine and methylamine (MMA) were observed throughout the summer‐long study, increasing during a period coinciding with a marked decline in Chl a , suggesting a lytic event led to their release; however, this did not correspond to increased CH 4 concentrations. Spiking river water with GB or MMA yielded significantly greater CH 4 than nonspiked controls, illustrating the metabolic potential of the river microbiome. In summary, this study provides evidence that: (1) phototrophic microorganisms are involved in CH 4 synthesis in a river environment; (2) the river microbiome possesses the metabolic potential to convert methylated amines to CH 4 ; and (3) river CH 4 concentrations are dynamic diurnally as well as during the summer active months.