Multiplex digital PCR with digital melting curve analysis on a self-partitioning SlipChip
Yan Yu, Ziqing Yu, Xufeng Pan, Lei Xu, Rui Guo, Xiaohua Qian, Feng Shen
Abstract
. Since digital MCA only requires an intercalation dye instead of sequence-specific hydrolysis probes to perform multiplex digital PCR analysis, it can be less expensive and not limited to the number of fluorescence channels.
Topics & Concepts
AmpliconDigital polymerase chain reactionMultiplexMelting curve analysisChemistryPolymerase chain reactionBioinformaticsBiologyGeneBiochemistryBiosensors and Analytical DetectionInnovative Microfluidic and Catalytic Techniques InnovationMolecular Biology Techniques and Applications