A novel streptonigrin type alkaloid from the <i>Streptomyces flocculus</i> CGMCC 4.1223 mutant Δ<i>stnA/Q2</i>
Xiaozheng Wang, Fei Xu, Tingting Huang, Zixin Deng, Shuangjun Lin
Abstract
Streptonigrin (STN) is a highly functionalized aminoquinone alkaloid with broad and potent antitumor activities. Previously, the biosynthetic gene cluster of STN was identified in Streptomyces flocculus CGMCC 4.1223, revealing an α/β-hydrolase (StnA) and a methyltransferase (StnQ2). In this work, a double mutant ΔstnA/Q2 was constructed by genetic manipulation and produced a novel derivative of STN, named as streptonigramide. Structure of streptonigramide was established by spectroscopic analyses. Its biosynthetic pathway has been proposed as well.
Topics & Concepts
MutantChemistryDerivative (finance)StereochemistryAlkaloidGene clusterStreptomycesBiochemistryBiologyGeneGeneticsBacteriaEconomicsFinancial economicsMicrobial Natural Products and BiosynthesisCarbohydrate Chemistry and SynthesisPhytochemical compounds biological activities