Microbial profiling of peri-implantitis compared to the periodontal microbiota in health and disease using 16S rRNA sequencing
Hyun‐Joo Kim, Dae-Hee Ahn, Yeuni Yu, Hyejung Han, Si Yeong Kim, Ji‐Young Joo, Jin Chung, Hee Sam Na, Ju Youn Lee
Abstract
PURPOSE: The objective of this study was to analyze the microbial profile of individuals with peri-implantitis (PI) compared to those of periodontally healthy (PH) subjects and periodontitis (PT) subjects using Illumina sequencing. METHODS: Buccal, supragingival, and subgingival plaque samples were collected from 109 subjects (PH: 30, PT: 49, and PI: 30). The V3-V4 region of 16S rRNA was sequenced and analyzed to profile the plaque microbiota. RESULTS: . Network analysis identified that several well-known periodontal pathogens and newly recognized bacteria were closely correlated with each other. CONCLUSIONS: The composition of the microbiota was considerably different in PI subjects compared to PH and PT subjects, and these results could shed light on the mechanisms involved in the development of PI.