Harnessing galactose oxidase in the development of a chemoenzymatic platform for glycoconjugate vaccine design
Jeremy A. Duke, Amy V. Paschall, John Glushka, Andrew Lees, Kelley W. Moremen, Fikri Y. Avci
Abstract
illustrates the functionally deleterious role chemical oxidation can have on CPS structures. Immunization with the conjugate synthesized using GOase provided a markedly improved humoral response over the traditional periodate-oxidized group. Further, functional protection was validated in vitro by measure of opsonophagocytic killing and in vivo through a lethality challenge in mice. Overall, this work introduces a strategy for glycoconjugate development that overcomes limitations previously known to play a role in the current approach of vaccine design.
Topics & Concepts
GlycoconjugateConjugatePeriodateSodium periodateBioconjugationChemistryGalactose oxidaseBiochemistryCombinatorial chemistryBioorthogonal chemistryDerivatizationGalactosePneumococcal conjugate vaccineStreptococcus pneumoniaeOrganic chemistryAntibioticsClick chemistryMathematical analysisHigh-performance liquid chromatographyMathematicsPneumonia and Respiratory InfectionsCarbohydrate Chemistry and SynthesisBacterial Infections and Vaccines