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SLAMF8 promotes the proliferation and migration of synovial fibroblasts by regulating the ERK/MMPs signalling pathway

Jun Liu, Ying Huang, Jiashun Zeng, Changming Chen, Peiting Li, Qiaoyi Ning, Xianyue Guan, Long Li

2022Autoimmunity14 citationsDOI

Abstract

wound healing assay and transwell migration assay. In the present study, after TNF-α treatments, the SLAMF8 mRNA and protein expression in both MH7A and HFLS-RA cell lines have a time-dependent increase. The attenuation of SLAMF8 ameliorated TNF-α-induced proliferation, invasion and migration in MH7A and HFLS-RA cells. Simultaneously, when SLAMF8 was silenced, the expression of p-ERK, MMP-1, and MMP-13 was suppressed significantly. In summary, these results indicated that the knockdown of the SLAMF8 significantly attenuated TNF-α-induced proinflammatory responses in MH7A and HFLS-RA cells. Therefore, SLAMF8 exhibits therapeutic potential for the management of inflammation in rheumatoid arthritis.

Topics & Concepts

Proinflammatory cytokineRheumatoid arthritisGene knockdownCancer researchInflammationMAPK/ERK pathwayCell migrationApoptosisCell growthFibroblastAnnexinArthritisSynovial membraneTumor necrosis factor alphaImmunologyChemistryMedicineCell cultureCellCell biologySignal transductionBiologyFlow cytometryGeneticsBiochemistryRheumatoid Arthritis Research and TherapiesCytokine Signaling Pathways and InteractionsSignaling Pathways in Disease
SLAMF8 promotes the proliferation and migration of synovial fibroblasts by regulating the ERK/MMPs signalling pathway | Litcius