Substitution of PINK1 Gly411 modulates substrate receptivity and turnover
Fabienne C. Fiesel, Dominika Fričová, Caleb S Hayes, Matt Coban, Roman Hudec, Jenny M. Bredenberg, Benjamin J. Broadway, Briana N Markham, Tingxiang Yan, Paige K Boneski, Gabriella Fiorino, Jens O. Watzlawik, Xu Hou, Arthur M McCarty, Laura J. Lewis‐Tuffin, Jun Zhong, Benjamin J. Madden, Alban Ordureau, Heeseon An, Andreas Puschmann, Zbigniew K. Wszołek, Owen A. Ross, J. Wade Harper, Thomas R. Caulfield, Wolfdieter Springer
Abstract
: ATP: adenosine triphosphate; CCCP: carbonyl cyanide m-chlorophenyl hydrazone; Ub-CR: ubiquitin with C-terminally retracted tail; CTD: C-terminal domain (of PINK1); ELISA: enzyme-linked immunosorbent assay; HCI: high-content imaging; IB: immunoblot; IF: immunofluorescence; NPC: neuronal precursor cells; MDS: molecular dynamics simulation; PD: Parkinson disease; p-S65-Ub: ubiquitin phosphorylated at Ser65; RMSF: root mean scare fluctuation; TOMM: translocase of outer mitochondrial membrane; TVLN: ubiquitin with T66V and L67N mutation, mimics Ub-CR; Ub: ubiquitin; WT: wild-type.