Quantification of NADH:ubiquinone oxidoreductase (complex I) content in biological samples
Fariha Ansari, Belem Yoval‐Sánchez, Zoya Niatsetskaya, Sergey A. Sosunov, Анна Степанова, Christian Garcia, Edward Owusu-Ansah, Vadim S. Ten, Ilka Wittig, Alexander Galkin
Abstract
complex I molecules are present in a single human embryonic kidney 293 cell. The ability to determine complex I content should provide a valuable tool to investigate the enzyme status in samples after in vivo treatment in mutant organisms, cells in culture, or human biopsies.
Topics & Concepts
MitochondrionBiochemistryElectron Transport Complex IOxidoreductaseBiologyEnzymeNADH dehydrogenaseNAD+ kinaseMitochondrial DNAMolecular biologyGeneMitochondrial Function and PathologyMetabolism and Genetic DisordersMetalloenzymes and iron-sulfur proteins