Low Overpotential Amperometric Sensor Using Yb2O3.CuO@rGO Nanocomposite for Sensitive Detection of Ascorbic Acid in Real Samples
Jahir Ahmed, M. Faisal, Jari S. Algethami, Mabkhoot Alsaiari, Saeed A. Alsareii, Farid A. Harraz
Abstract
The ultimate objective of this research work is to design a sensitive and selective electrochemical sensor for the efficient detection of ascorbic acid (AA), a vital antioxidant found in blood serum that may serve as a biomarker for oxidative stress. To achieve this, we utilized a novel Yb2O3.CuO@rGO nanocomposite (NC) as the active material to modify the glassy carbon working electrode (GCE). The structural properties and morphological characteristics of the Yb2O3.CuO@rGO NC were investigated using various techniques to ensure their suitability for the sensor. The resulting sensor electrode was able to detect a broad range of AA concentrations (0.5–1571 µM) in neutral phosphate buffer solution, with a high sensitivity of 0.4341 µAµM−1cm−2 and a reasonable detection limit of 0.062 µM. The sensor’s great sensitivity and selectivity allowed it to accurately determine the levels of AA in human blood serum and commercial vitamin C tablets. It demonstrated high levels of reproducibility, repeatability, and stability, making it a reliable and robust sensor for the measurement of AA at low overpotential. Overall, the Yb2O3.CuO@rGO/GCE sensor showed great potential in detecting AA from real samples.