Group 3 innate lymphocytes make a distinct contribution to type 17 immunity in bladder defence
Alexandra Riding, Kevin W. Loudon, Andrew Guo, John R. Ferdinand, Laurence S. C. Lok, Nathan Richoz, Andrew P. Stewart, Tomas Castro‐Dopico, Zewen Kelvin Tuong, Rémi Fiancette, Georgina Bowyer, Aaron Fleming, Eleanor S. Gillman, Ondřej Suchánek, Krishnaa T. Mahbubani, Kourosh Saeb-Parsy, David R. Withers, Gordan Dougan, Simon Clare, Menna R. Clatworthy
Abstract
mice, implicating group 3 innate lymphoid cells (ILC3s) as a source of these cytokines. NCR-positive and negative ILC3 subsets were identified in murine and human bladders, with local proliferation increasing IL17-producing ILC3s post infection. ILC3s made a more limited contribution to bladder IL22, with prominent early induction of IL22 evident in Th17 cells. Single-cell RNA sequencing revealed bladder NCR-negative ILC3s as the source of IL17 and identified putative ILC3-myeloid cell interactions, including via lymphotoxin-β-LTBR. Altogether, our data provide important insights into the orchestration and execution of type 17 immunity in bladder defense.