Mechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes
Yuki Sasaki, Masahiro Komeno, Akihiro Ishiwata, Ayako Horigome, Toshitaka Odamaki, Jin‐zhong Xiao, Katsunori Tanaka, Yukishige Ito, Kanefumi Kitahara, Hisashi Ashida, Kiyotaka Fujita
Abstract
Terminal galactose residues of the side chain of gum arabic arabinogalactan protein (AGP) are mainly substituted by α1,3/α1,4-linked Ara f and β1,6-linked α- l -Rha p -(1→4)-β- d -Glc p A residues. This study found a multidomain BlArafE with GH43_22 and GH43_34 catalytic domains showing cooperative action for degrading α1,3/α1,4-linked Ara f of the side chain of gum arabic AGP.
Topics & Concepts
ArabinogalactanBifidobacterium longumGum arabicBiochemistryTetrasaccharideGlycoside hydrolaseOligosaccharideEnzymeBiologyBifidobacteriumLactoseGalactosePolysaccharideFermentationChemistryFood scienceLactobacillusEnzyme Production and CharacterizationPolysaccharides and Plant Cell WallsMicrobial Metabolites in Food Biotechnology