An ancestral molecular response to nanomaterial particulates
Giusy del Giudice, Angela Serra, Laura Aliisa Saarimäki, Konstantinos Kotsis, Ian Rouse, Stefana Anais Colibaba, Karolina Jagiełło, Alicja Mikołajczyk, Michele Fratello, Anastasios G. Papadiamantis, Natasha Sanabria, Maria Emilia Annala, Jack Morikka, Pia Kinaret, Evangelos Voyiatzis, Georgia Melagraki, Antreas Afantitis, Kaido Tämm, Tomasz Puzyn, Mary Gulumian, Vladimir Lobaskin, Iseult Lynch, Antonio Federico, Dario Greco
Abstract
Abstract The varied transcriptomic response to nanoparticles has hampered the understanding of the mechanism of action. Here, by performing a meta-analysis of a large collection of transcriptomics data from various engineered nanoparticle exposure studies, we identify common patterns of gene regulation that impact the transcriptomic response. Analysis identifies deregulation of immune functions as a prominent response across different exposure studies. Looking at the promoter regions of these genes, a set of binding sites for zinc finger transcription factors C 2 H 2 , involved in cell stress responses, protein misfolding and chromatin remodelling and immunomodulation, is identified. The model can be used to explain the outcomes of mechanism of action and is observed across a range of species indicating this is a conserved part of the innate immune system.