Short-wavelength, ultrafast thulium-doped fiber laser system for three-photon microscopy
Yutaka Nomura, Hideji Murakoshi, Takao Fuji
Abstract
An ultrafast, thulium-doped fiber laser system is developed for three-photon microscopy. The system generates 150 fs pulses at the center wavelength of 1.82 µm with a pulse energy of 1.1 µJ at the repetition rate of 1 MHz. The generated pulses are applied to a three-photon fluorescence microscope, with which biological samples expressing red fluorescent proteins are observed through three-photon excitation processes.
Topics & Concepts
Ultrashort pulseMaterials scienceTwo-photon excitation microscopyThuliumOpticsLaserFiber laserFluorescence microscopeMicroscopyFluorescenceWavelengthMicroscopeOptoelectronicsPulse (music)PhotonDopingPhysicsDetectorAdvanced Fluorescence Microscopy TechniquesAdvanced Fiber Laser TechnologiesOptical Coherence Tomography Applications