Targeted mutagenesis in Arabidopsis and medicinal plants using transposon‐associated TnpB
Zongyou Lv, Wen‐Hua Chen, Wen‐Hua Chen, Shiyuan Fang, Boran Dong, Xingxing Wang, Lida Zhang, Jing‐Shi Xue, Wansheng Chen, Wansheng Chen
Abstract
The programmable nuclease TnpB is significantly smaller than Cas9, can edit genes in medicinal plants, including Artemisia annua, Salvia miltiorrhiza, Scutellaria baicalensis, Isatis indigotica, and Codonopsis pilosula, and has potential uses in molecular breeding to enhance crop yield and quality.
Topics & Concepts
Genome editingBiologyCas9CRISPRArabidopsisGenomeGeneticsTransposable elementComputational biologyTransposon mutagenesisGeneMutagenesisMutantCRISPR and Genetic EngineeringRNA regulation and diseaseChromosomal and Genetic Variations