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Safety and improved efficacy signals following gene therapy in childhood blindness caused by GUCY2D mutations

Samuel G. Jacobson, Artur V. Cideciyan, Allen C. Ho, Igor V. Peshenko, Alexandra V. Garafalo, Alejandro J. Román, Alexander Sumaroka, Vivian Wu, Arun K. Krishnan, Rebecca Sheplock, Sanford L. Boye, Bee-Lin Cheang, Vanessa Davidson, Catherine R. O’Riordan, Alexander M. Dizhoor, Sanford L. Boye

2021iScience45 citationsDOIOpen Access PDF

Abstract

gene was delivered by subretinal injection to one eye in three adult patients with severe visual loss, nystagmus, but preserved retinal structure. Safety and efficacy parameters were monitored for 9 months post-operatively. No systemic toxicity was detected; there were no serious adverse events, and ocular adverse events resolved. P1 and P2 showed statistically significant rod photoreceptor vision improvement by full-field stimulus testing in the treated eye. P1 also showed improvement in pupillary responses. Visual acuity remained stable from baseline in P1 and P2. P3, however, showed a gain of 0.3 logMAR in the treated eye, indicating greater cone-photoreceptor function. The results show safety and both rod- and cone-mediated efficacy of this therapy.

Topics & Concepts

GUCY2DAdverse effectGenetic enhancementNystagmusVisual acuityOphthalmologyRetinalMedicineBiologyGeneGeneticsAudiologyInternal medicineGuanylate cyclase 2CGuanylate cyclaseNitric oxideRetinal Development and DisordersRetinal Diseases and TreatmentsRetinal and Optic Conditions
Safety and improved efficacy signals following gene therapy in childhood blindness caused by GUCY2D mutations | Litcius