Multicompartmental analysis of the murine pulmonary immune response by spectral flow cytometry
Mary Y. Chang, J Brune, Michele Black, William A. Altemeier, Charles W. Frevert
2023American Journal of Physiology-Lung Cellular and Molecular Physiology13 citationsDOIOpen Access PDF
Abstract
We describe a novel combination of methodologies that incorporates dual in vivo compartmental analysis using intravascular and intratracheal CD45 labeling, a broad panel of antibodies for identifying lymphoid and nonlymphoid cells, and spectral flow cytometry that uses cellular autofluorescence to aid in resolving and identifying immune cell populations. This methodology allows precise localization of immune cells in the lavageable airways, nonlavageable airways, interstitial lung tissue, and marginated in the lung vasculature.
Topics & Concepts
Flow cytometryAutofluorescenceImmune systemImmunologyBiologyIn vivoInflammationLungAntibodyCell typePathologyComputational biologyCellMedicineQuantum mechanicsGeneticsFluorescenceInternal medicinePhysicsBiotechnologyIL-33, ST2, and ILC PathwaysImmune cells in cancerNeonatal Respiratory Health Research