Improving thermo-tolerance of <i>Saccharomyces cerevisiae</i> by precise regulation of the expression of small HSP
Meiling Zhang, Hui Zhang, Ya-xin He, Zhao‐Hui Wu, Ke Xu
Abstract
t), the two best performing engineered strains, exhibited a 19.8% and 17.2% increase in cell density, respectively, compared to the control strain. Additionally, the analysis of pyruvate kinase (PK) and malate dehydrogenase (MDH) enzyme activities indicated that the engineered strains enhanced protein quality at higher temperatures. The research methods and ideas presented in this paper have significant scientific reference value for exploring and applying other stress-resistant gene circuits.
Topics & Concepts
Saccharomyces cerevisiaeCell biologyExpression (computer science)ChemistryComputational biologyBiochemistryBiologyComputer scienceYeastProgramming languageHeat shock proteins researchViral Infectious Diseases and Gene Expression in InsectsGenetics, Aging, and Longevity in Model Organisms