Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA
Qiao-Jun Zhang, Yue Liu, Wenting Zhang, Jingjing Huang, Hao‐Hong Li, Youguang Lu, Ming Zheng, Dali Zheng
Abstract
Objective: To synthesize and determine the antifungal activity of AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) against Candida albicans ( C. albicans ) for use in the field of denture cleaning. Methods: The morphology and structure of [email protected] were characterized by IR, UV-Vis, XRD and SEM. The antifungal potential of [email protected] against C. albicans was determined by colony formation assay and growth curve analysis. PMMA containing [email protected] was prepared, and the long-term antifungal efficacy was analyzed. The effect against C. albicans biofilm was analyzed by SEM and OD600 , and the color changes of the specimens were observed by stereomicroscopy after 1 week of incubation. Cytotoxicity to human oral gingival fibroblasts and oral mucosal epithelial cells was detected by Cell Counting Kit-8 (CCK-8) in vitro. Results: The compound showed a good crystalline phase, the presence of AgBr nanoparticles and the hybridization of CTMAB+ with AgBr-NPs. [email protected] showed significant antifungal activity against C. albicans at concentrations of 10 μg/mL and 20 μg/mL. PMMA specimens containing [email protected] showed no long-term antifungal effect against C. albicans biofilm. The clearance rate of C. albicans attached to PMMA was 44.73% after soaking in 10 μg/mL [email protected] solution for 30 min and 91.35% for 8 h. There was no significant residual cytotoxicity or visual color change after soaking. Significance: [email protected] showed promising potential treatment for denture cleaners. Keywords: C. albicans , [email protected] , polymethyl methacrylate, cytotoxicity, biofilm