Anticancer activity of Green synthesized selenium nanoparticles from Garcinia Mangostana Crude extract against MCF-7 Breast cancer cells
Georgia Benithaj, Pratibha Ramani, Rajesh Kumar, Kartikeyan Ramalingam, Abilasha Ramasubramaniam, Global Burden Of Disease, ; Kocarnik, J Compton, K Dean, F Fu, W Gaw, B, M Mathew, S Samuel, A Soni, K Roopa, B Princeton, P Santhakumar, L Prathap, M Rayman, A Tugarova, P Mamchenkova, Y Dyatlova, A Kamnev, H Winkler, M Suter, H Naegeli, A Yadav, U Gupta, R Sharma, Y Zhou, M Xu, Y Liu, Y Bai, Y Deng, J Liu, X Wang, K Sun, Y Tan, S Wu, J Zhang, M Rayman, C Sanmartn, D Plano, A Sharma, J Palop, S Fazal, A Jayasree, S Sasidharan, M Koyakutty, S Nair, D Menon, A Genevieve, M Kahrilas Laura, S Wally, H Fredrick Michael, L Amy, J Prieto, Owens, C Ramamurthy, K Sampath, P Arunkumar, M Suresh Kumar, V Sujatha, K Premkumar, C Thirunavukkarasu, D Suresh, P Nethravathi, Udayabhanu, H Rajanaika, H Nagabhushana, S Sharma, Raj Preeth, D Saravanan, S Shairam, M Selvakumar, N, Selestin Raja, I Dhanasekaran, A, G Bauelos, Z Lin, D Liang, X Yin, S Bajpai, P Shukla, M Adil, S Asiedu, K Pruski, B Prithiviraj, M Prithiviraj, R Muralikannan, P Moongkarndi, N Kosem, S Kaslungka, O Luanratana, N Pongpan, N Neungton, L Kalick, H Khan
Abstract
Background: Nano-biotechnology has evolved and plays a crucial role in the detection and treatment of many diseases in the current scenario.An eco-friendly and efficient method (photoinduced) has been used for green synthesis of stable selenium nanoparticles (SeNPs) using aqueous extract as a reducing and stabilizing agent, derived from fruit extract of garcinia mangostana.Aim: The aim of this study was to evaluate the anticancer activity of green synthesized GM selenium nanoparticles (Se-NPs) against breast cancer cells (MCF-7).Methods And Materials: The preparation of selenium-induced Garcinia Mangostana nanoparticles were examined by ultraviolet-visible spectroscopy with a wavelength of range from 25-650nm.The selenium nanoparticle pellet was stored in sealed vials after being calcined for two hours at 70°C in a hot air oven.In-vitro antitumor activity of Se-NPs was evaluated by MTT assay.Inverted microscope was used to evaluate the morphology of the cells. Results:The synthesized Selenium Nanoparticles have anticancer against MCF-7 cell lines.The cytotoxicity study exhibited a dose-dependent effect against breast cancer cells (MCF-7) using MTT assay, the inhibitory concentration (IC50) was found to be 50μg/mL .Inverted phase contrast microscope showed low viability of cell at a concentration of 60ul in the breast cancer cell line. Conclusion:In conclusion, the results of this study demonstrated that Garcinia Mangostana induced selenium Nanoparticles extract may be a potential therapeutic agent for human breast cancer treatment.e75