Antimicrobial resistance of Escherichiacoli and Salmonella isolated from retail broiler chicken carcasses in Zambia
Elizabeth Muligisa-Muonga, Geoffrey Mainda, Mercy Mukuma, Geoffrey Kwenda, Bernard M. Hang’ombe, Flavien Nsoni Bumbangi, Nelson Phiri, Mwaba Mwansa, Musso Munyeme, John Bwalya Muma
Abstract
Antimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries such as Zambia. This study was undertaken to determine the antimicrobial resistance profiles of Escherichia coli (E. coli) and Salmonella isolated from retail broiler chicken carcasses purchased from open markets and supermarkets in Zambia. A total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates were done using Analytical Profile Index (API 20E) (Biomerieux®) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 9 antibiotics. WHONET 2018 software was used to analyze AST results. The E. coli isolates were mostly resistant to tetracycline (79.4%), ampicillin (51.9%), and trimethoprim/sulfamethoxazole (49.7%). Two of the five Salmonella isolates were resistant to at least one antibiotic. This study has demonstrated the presence of AMR E. coli and Salmonella on retail broiler chicken carcasses from open markets and supermarkets, which is of public health concern.