Discovery of a polymer resistant to bacterial biofilm, swarming, and encrustation
Jean‐Frédéric Dubern, Andrew L. Hook, Alessandro M. Carabelli, Chien‐Yi Chang, C Lewis-Lloyd, Jeni Luckett, Laurence Burroughs, Adam A. Dundas, David J. Humes, Derek J. Irvine, Morgan R. Alexander, Paul Williams
Abstract
Innovative approaches to prevent catheter-associated urinary tract infections (CAUTIs) are urgently required. Here, we describe the discovery of an acrylate copolymer capable of resisting single- and multispecies bacterial biofilm formation, swarming, encrustation, and host protein deposition, which are major challenges associated with preventing CAUTIs. After screening ~400 acrylate polymers, poly( tert -butyl cyclohexyl acrylate) was selected for its biofilm- and encrustation-resistant properties. When combined with the swarming inhibitory poly(2-hydroxy-3-phenoxypropyl acrylate), the copolymer retained the bioinstructive properties of the respective homopolymers when challenged with Proteus mirabilis , Pseudomonas aeruginosa , Staphylococcus aureus , and Escherichia coli . Urinary tract catheterization causes the release of host proteins that are exploited by pathogens to colonize catheters. After preconditioning the copolymer with urine collected from patients before and after catheterization, reduced host fibrinogen deposition was observed, and resistance to diverse uropathogens was maintained. These data highlight the potential of the copolymer as a urinary catheter coating for preventing CAUTIs.