Rapid Identification of Methicillin-Resistant Staphylococcus aureus Using MALDI-TOF MS and Machine Learning from over 20,000 Clinical Isolates
Jiaxin Yu, Ni Tien, Yu‐Ching Liu, Der‐Yang Cho, Jiawen Chen, Yin-Tai Tsai, Yu-Chen Huang, Huei-Jen Chao, Chao‐Jung Chen
Abstract
Over 20,000 clinical MSSA and MRSA isolates were collected to build a machine learning (ML) model to identify MSSA/MRSA and their markers. This model was tested across four external clinical sites to ensure the model's usability. We report the first discovery and validation of MRSA markers on the largest scale of clinical MSSA and MRSA isolates collected to date, covering five different clinical sites. Our developed approach for the rapid identification of MSSA and MRSA can be highly integrated into the current workflows.
Topics & Concepts
Staphylococcus aureusUsabilityIdentification (biology)Methicillin-resistant Staphylococcus aureusMedicineArtificial intelligenceMicrobiologyComputer scienceComputational biologyBiologyBacteriaGeneticsHuman–computer interactionEcologyBacterial Identification and Susceptibility TestingAntimicrobial Resistance in StaphylococcusStreptococcal Infections and Treatments