Ultrasensitive detection of gene-PIK3CAH1047R mutation based on cascaded strand displacement amplification and trans-cleavage ability of CRISPR/Cas12a
Yuanyi Deng, Gaihua Cao, Xiaolong Chen, Mei Yang, Danqun Huo, Changjun Hou
Topics & Concepts
ChemistryCRISPRMultiple displacement amplificationCleavage (geology)Molecular biologyGenePolymerase chain reactionBiochemistryDNA extractionGeotechnical engineeringBiologyFracture (geology)EngineeringCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniques