Characterization of ultrasonically extracted flaxseed polysaccharide gum and assessing its lipid‐lowering potential in a rat model
Muhammad Nadeem Akhtar, Anees Ahmed Khalil, Ahmed Bilal, Muhammad Afzaal, Tabussam Tufail, Rabia Saeed, Rabia Siddique, Arash Nemat, Muhammad Faisal Manzoor
Abstract
Abstract Flaxseed polysaccharide gum (FPG) was extracted through the ultrasound‐assisted process using water as a solvent with a yield ranging from 8.05 ± 0.32% to 12.23 ± 0.45% by changing different extraction variables. The extracted FPG was analyzed for its functional groups and antioxidant potential. The maximum DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) free radical scavenging activity (≈100%) of FPG was noted at concentrations beyond ≈10 mg·ml −1 . The maximum inhibition percentage through ABTS (2,2′‐azino‐bis 3‐ethylbenzothiazoline‐6‐sulfonic acid) (72.4% ± 1.9%) was noted at 40 mg·ml −1 , which was observed to be less when compared to DPPH at the same concentration. The total antioxidant potential of the FPG solution at a concentration of 10 mg·ml −1 was equivalent to 461 mg ascorbic acid, which tends to increase with concentration at a much lower scope. The in vivo trial suggested that the least weight gain was noted in experimental groups G 2 and Gh 2 . A significant reduction in total cholesterol was noticed in G 1 (−14.14%) and G 2 (−17.72%) and in Gh 1 (−22.02%) and Gh 2 (−34.68%) after 60 days of the trial compared to the baseline values. The maximum reduction in total triglyceride was observed in Gh 2 (−25.06%) and Gh 1 (−22.01%) after 60 days of the trial. It was an increasing trend in high‐density lipoprotein cholesterol (HDL‐c) in different experimental groups G 2 (10.51%) than G 1 (5.35%) and Gh 2 (48.96%) and Gh 1 (31.11%), respectively, after 60 days of study interval. Reduction of −5.05% and − 9.45% was observed in G 1 and G 2 , while similar results were observed in Gh 1 and Gh 2 . Conclusively, results suggested a possible protective role of FPG against hyperlipidemia.