A novel integrated bio-reactor of moving bed and constructed wetland (MBCW) for domestic wastewater treatment and its microbial community diversity
Changmiao Lai, Yu Sun, Yong Guo, Qin Cai, Ping Yang
Abstract
An MBBR and CW combo bio-reactor (MBCW) was designed as a novel hybrid process for simultaneous organic, nitrogen and phosphate removal through the long-term operation. The effect of the internal recycling rate (IRR), hydraulic retention time (HRT) and chemical oxygen demand/total nitrogen (C/N) ratio were all discussed, and the recommended values were 5:1, 12 h and >6, respectively. A higher C/N ratio was a key factor for achieving a higher TN removal. The mixed biocarrier system was realized by inoculating porous polymer carriers (PPC) and cylindrical polyethylene carriers (CPC) and achieving a higher organic biodegradation and nitrification rate compared to a single carrier system. Microorganism activities and plants’ uptake or utilization both contributed to the nutrient removal in a constructed wetland. High-throughput sequencing results revealed an abundant microbial diversity and a distinct microbial distribution in the whole system where Flavobacterium (14.2%), Acinetobacter (12.87%) and Rhodobacter (10.83%) dominated on PPC, Terrimonas (8.88%), Reyranella (6.61%) and Rubinisphaera (5.63%) dominated on CPC, Comamonas (4.18%), Gemmobacter (4.02%) and Hydrogenophaga (3.97%) dominated on CWs, as well as Citrobacter (53.13%) on suspended floc.