Topological analyses of the L-lysine exporter LysO reveal a critical role for a conserved pair of intramembrane solvent-exposed acidic residues
Swati Dubey, Puja Majumder, Aravind Penmatsa, Abhijit A. Sardesai
Abstract
and that Lys may be a low-affinity export substrate. Our findings are compatible with a physiological scenario wherein in vivo LysO exports the naturally occurring antimetabolite Thl with higher affinity over the essential cellular metabolite Lys, thus affording protection from Thl toxicity and limiting wasteful export of Lys.
Topics & Concepts
Membrane topologyBiochemistryTransmembrane domainFunction (biology)LysineBiologyTransmembrane proteinLyso-ChemistryCell biologyStereochemistryMembraneAmino acidReceptorElectrical engineeringScintillatorDetectorEngineeringBacterial Genetics and BiotechnologyAmino Acid Enzymes and MetabolismDrug Transport and Resistance Mechanisms