Nanomaterial Size and Surface Modification Mediate Disease Resistance Activation in Cucumber (<i>Cucumis sativus</i>)
Xing Luo, Zhenyu Wang, Chuanxi Wang, Le Yue, Mengna Tao, Wade H. Elmer, Jason C. White, Xuesong Cao, Baoshan Xing
Abstract
Crop disease represents a serious and increasing threat to global food security. Lanthanum oxide nanomaterials (La 2 O 3 NMs) with different sizes (10 and 20 nm) and surface modifications (citrate, polyvinylpyrrolidone [PVP], and poly(ethylene glycol)) were investigated for their control of the fungal pathogen Fusarium oxysporum (Schl.) f. sp cucumerinum Owen on six-week-old cucumber ( Cucumis sativus ) in soil. Seed treatment and foliar application of the La 2 O 3 NMs at 20–200 mg/kg (mg/L) significantly suppressed cucumber wilt (decreased by 12.50–52.11%), although the disease control efficacy was concentration-, size-, and surface modification-dependent. The best pathogen control was achieved by foliar application of 200 mg/L PVP-coated La 2 O 3 NMs (10 nm); disease severity was decreased by 67.6%, and fresh shoot biomass was increased by 49.9% as compared with pathogen-infected control. Importantly, disease control efficacy was 1.97- and 3.61-fold greater than that of La 2 O 3 bulk particles and a commercial fungicide (Hymexazol), respectively. Additionally, La 2 O 3 NMs application enhanced cucumber yield by 350–461%, increased fruit total amino acids by 295–344%, and improved fruit vitamin content by 65–169% as compared with infected controls. Transcriptomic and metabolomic analyses revealed that La 2 O 3 NMs: (1) interacted with calmodulin, subsequently activating salicylic acid-dependent systemic acquired resistance; (2) increased the activity and expression of antioxidant and related genes, thereby alleviating pathogen-induced oxidative stress; and (3) directly inhibited in vivo pathogen growth. The findings highlight the significant potential of La 2 O 3 NMs for suppressing plant disease in sustainable agriculture.