Highly selective two-photon fluorescent off–on probes for imaging tyrosinase activity in living cells and tissues
Yixin Cui, Sang Jun Park, Xiaofeng Wu, Rui Wang, Sujie Qi, Hwan Myung Kim, Juyoung Yoon
Abstract
A coumarin-based two-photon (TP) fluorescent off-on probe has been developed for detecting tyrosinase activity. High selectivity, sensitivity and biocompatibility enable the probes to successfully image tyrosinase activity in live cells and tissues using TP microscopy.
Topics & Concepts
FluorescenceTyrosinaseTwo-photon excitation microscopyChemistryBiophysicsBiochemistryBiologyEnzymeOpticsPhysicsmelanin and skin pigmentationPhotochromic and Fluorescence ChemistryBiochemical Analysis and Sensing Techniques