Stress and timing associated with Caenorhabditis elegans immobilization methods
Jacob R. Manjarrez, Roger Mailler
Abstract
BACKGROUND: to be immobilized for imaging or laser ablation experiments. There are a number of known techniques for immobilizing worms, but to our knowledge, there are no comprehensive studies of the various agents in common use today. NEW METHOD: stress reporters. RESULTS: reporter. COMPARISON WITH EXISTING METHODS: This study shows that commonly used concentrations of immobilizing agents are ineffective when evaporation is prevented. CONCLUSIONS: To improve reproducibility of results it is essential to use consistent concentrations of immobilizing agents. It is also critically important to account for stress-related after effects elicited by immobilization agents when designing any experiment.
Topics & Concepts
Sodium azideCaenorhabditis elegansChemistryBiophysicsReducing agentBiologyBiochemistryGeneOrganic chemistryGenetics, Aging, and Longevity in Model OrganismsSpaceflight effects on biologythermodynamics and calorimetric analyses