<scp>OMIP‐91</scp>: A 27‐color flow cytometry panel to evaluate the phenotype and function of human conventional and unconventional T‐cells
Jeremy Anderson, Sedi Jalali, Paul V. Licciardi, Daniel G. Pellicci
Abstract
This 27-color panel was developed to simultaneously measure different T-cell populations (CD4, CD8, γδ T-cells, and MAIT cells) and their subsets (Memory, Th1, Th2, Th17, Tfh, and Treg) along with functional markers associated with their activation status, cytokine production and cytotoxicity. This panel will be useful for both in vivo and in vitro studies evaluating T-cells in the context of human health and disease. This panel is valuable in settings where samples are limited as a large amount of data will be generated using small volumes of blood.
Topics & Concepts
Flow cytometryContext (archaeology)PhenotypeImmunologyCytokineCD8CytometryCytotoxicityCytotoxic T cellIn vitroFunction (biology)In vivoBiologyCell biologyImmune systemBiochemistryBiotechnologyGenePaleontologyImmune Cell Function and InteractionT-cell and B-cell ImmunologySingle-cell and spatial transcriptomics