Impedimetric Biosensor for Monitoring Complementary DNA from Hepatitis B Virus Based on Gold Nanocrystals
Mohsen Shariati
Abstract
A highly sensitive label free electrochemical impedance biosensor based on gold nanocrystals (AuNCs) for hepatitis B virus (HBV) detection in blood serum was reported. A functional platform for measuring the geno-biosensor sensitivity for detecting HBV DNA based on AuNCs electrode was materialized. The adjusted morphologies of 50–100 nm beaded AuNCs on the In 2 O 3 support layer were materialized and characterized by a field emission scanning electron microscope (FESEM) and a high resolution transmission electron microscope (HRTEM). The bio-sensing measurements were conducted by electrochemical impedance spectroscopy (EIS) approach under redox reactions process. The HBV DNA probe (ssDNA) was immobilized on the AuNCs surface by forming a special bond between ssDNA and gold. The DNA target was diagnosed using ssDNA/AuNCs biosensor through EIS measurements in concentration ranges from 0.1 pM to 0.1 μ M with a limit of detection (LOD) of 0.1 fM. The selectivity property of the biosensor was investigated and it could distinctively distinguish the complementary DNA target from the non-complementary DNA, 1-, 2- and 3-mismatch targets. Finally, the capability of the electrode for detection of the HBV in blood serum samples was explored and biosensor showed interesting results in the HBV DNA sensing as a potential candidate for practical applications.