Multiplexed and amplified chemiluminescence resonance energy transfer (CRET) detection of genes and microRNAs using dye-loaded hemin/G-quadruplex-modified UiO-66 metal–organic framework nanoparticles
Pu Zhang, Yu Ouyang, Itamar Willner
Abstract
, the CRET processes in the hybrid nanostructures. These amplification platforms lead to the amplified sensing of miRNAs and genes. By mixing the Fl- and Rh 6G-loaded hairpin-functionalized UiO NMOFs, the multiplexed CRET detection of miRNA-155, miRNA-21 and the p53 and BRCA1 genes is demonstrated.
Topics & Concepts
HeminChemiluminescenceFörster resonance energy transferNanoparticleEnergy transfermicroRNADeoxyribozymeG-quadruplexChemistryMaterials scienceNanotechnologyFluorescencePhotochemistryGeneHemeDNAChromatographyDetection limitBiochemistryOpticsPhysicsEnzymeMolecular physicsAdvanced biosensing and bioanalysis techniquesMetal-Organic Frameworks: Synthesis and ApplicationsRNA Interference and Gene Delivery