Near-infrared-persistent luminescence/bioluminescence imaging tracking of transplanted mesenchymal stem cells in pulmonary fibrosis
Yuyang Xia, Hongying Bao, Jie Huang, Xiaodi Li, Chenggong Yu, Zhijun Zhang, Haishui Wang
Abstract
, were developed to track the dynamic translocation, position and distribution of the transplanted hMSCs, taking advantage of their long-lasting NIR-PL imaging ability and minimal autofluorescence background interference. Moreover, in virtue of their ability to express red-emitting firefly luciferase (RfLuc), the living stem cells after transplantation could be discriminated from the dead cells by BLI. This facile pattern contributes to the in situ monitoring of stem cells regarding their spontaneous behavior in vivo and therefore deepening our knowledge in the role played by the transplanted hMSCs in PF therapy.
Topics & Concepts
Bioluminescence imagingBioluminescenceStem cellMesenchymal stem cellLuminescenceInfraredChemistryCancer researchPathologyBiophysicsLuciferaseMedicineBiologyCell biologyMaterials scienceOpticsPhysicsOptoelectronicsBiochemistryGeneTransfectionInterstitial Lung Diseases and Idiopathic Pulmonary FibrosisInhalation and Respiratory Drug DeliveryChronic Obstructive Pulmonary Disease (COPD) Research