Machine learning-driven electronic identifications of single pathogenic bacteria
Shota Hattori, Rintaro Sekido, Iat Wai Leong, Makusu Tsutsui, Akihide Arima, Masayoshi Tanaka, Kazumichi Yokota, Takashi Washio, Tomoji Kawai, Mina Okochi
Abstract
A rapid method for screening pathogens can revolutionize health care by enabling infection control through medication before symptom. Here we report on label-free single-cell identifications of clinically-important pathogenic bacteria by using a polymer-integrated low thickness-to-diameter aspect ratio pore and machine learning-driven resistive pulse analyses. A high-spatiotemporal resolution of this electrical sensor enabled to observe galvanotactic response intrinsic to the microbes during their translocation. We demonstrated discrimination of the cellular motility via signal pattern classifications in a high-dimensional feature space. As the detection-to-decision can be completed within milliseconds, the present technique may be used for real-time screening of pathogenic bacteria for environmental and medical applications.