Improvement in the sensitivity of LA-ICP-MS bioimaging by addition of nitrogen to the argon carrier gas
Monique G. Mello, Thomas E. Lockwood, Jonathan Wanagat, Mika T. Westerhausen, David Bishop
Abstract
flow rate was determined for the analysis all elements and used to image endogenous elements in a mouse brain, and antibody-conjugated elements in a quadriceps muscle section. These images confirmed that the addition of N2 to the carrier gas increased the signal-to-background of the analysis, improving image resolution for endogenous elements and low abundant analytes used for immuno-mass spectrometry imaging of biomarkers. These findings offer a promising avenue for advancing the capabilities of LA-ICP-MS in bio-imaging applications.
Topics & Concepts
ArgonNitrogenArgon gasSensitivity (control systems)ChemistryNitrogen gasAnalytical Chemistry (journal)Inductively coupled plasma mass spectrometryMaterials scienceEnvironmental chemistryChromatographyMass spectrometryOrganic chemistryEngineeringElectronic engineeringMass Spectrometry Techniques and ApplicationsAnalytical chemistry methods developmentBiosensors and Analytical Detection