Highly parallel, wash-free, and ultrasensitive centrifugal droplet digital protein detection in sub-microliter blood
Zhengmin Tang, Feifei Lv, David Eun Reynolds, Shunji Zhang, Shufa Zheng, Jina Ko, Yu Chen, Yongcheng Wang
Abstract
A centrifugal droplet digital protein detection technology that combines miniaturized centrifugal droplet generation devices with digital immuno-PCR assays, achieving a femtomolar limit of detection of target proteins in sub-microliters of plasma.
Topics & Concepts
Detection limitCentrifugeProtein detectionDigital polymerase chain reactionChromatographyHuman plasmaRecombinant DNAChemistryBiomedical engineeringMaterials scienceNanotechnologyMedicinePolymerase chain reactionPhysicsBiochemistryNuclear physicsGeneInnovative Microfluidic and Catalytic Techniques InnovationBiosensors and Analytical DetectionMicrofluidic and Capillary Electrophoresis Applications