A stable biosensor for organophosphorus pesticide detection based on chitosan modified graphene
Jianhua Zhang, Huaying Hu, Pengchang Wang, Chi Zhang, Majiaqi Wu, Lianqiao Yang
Abstract
An acetylcholinesterase (AChE) biosensor was successfully fabricated with a stable structure and high detection accuracy. Graphene (Gra) nanofragments modified with chitosan (CS) and AChE were successively drip coated on the surface of a glassy carbon electrode via a layer-by-layer assembly method. The concentration range of the sensor to detect dichlorvos was 0.1-100,000 nM, and the limit of detection was 54 pM. CS was used to modify Gra for the first time, which enhanced the mechanical flexibility of these Gra nanostructures, significantly improving the stability and detection accuracy of this sensor.
Topics & Concepts
DichlorvosBiosensorDetection limitGrapheneChitosanMaterials scienceNanotechnologyLayer (electronics)ElectrodeAcetylcholinesteraseChromatographyChemistryChemical engineeringPesticideOrganic chemistryBiologyPhysical chemistryAgronomyEnzymeEngineeringElectrochemical sensors and biosensorsElectrochemical Analysis and ApplicationsCholinesterase and Neurodegenerative Diseases