Single-cell RNA sequencing reveals spatiotemporal heterogeneity and malignant progression in pancreatic neuroendocrine tumor
Yu Zhou, Si‐Yang Maggie Liu, Chao Liu, Jiabin Yang, Qing Lin, Shangyou Zheng, Changhao Chen, Quanbo Zhou, Rufu Chen
Abstract
Aims: Using Single-cell RNA sequencing (scRNA-seq), we explored the spatiotemporal heterogeneity of pancreatic neuroendocrine tumors (pNETs) and the underlying mechanism for malignant progression. Methods: scRNA-seq was conducted on three tumor tissues (two primary tissues from different sites, one liver metastatic lesion), one normal liver tissue, and peripheral blood mononuclear cells from one patient with a metastatic G2 pNET, followed by bioinformatics analysis and validation in a pNETs cohort.
Topics & Concepts
BiologyTranscriptomeCarcinogenesisTumor progressionNeuroendocrine tumorsCellCancer researchSingle-cell analysisPathologyMetastasisCancerGeneGene expressionMedicineGeneticsEndocrinologyNeuroendocrine Tumor Research AdvancesPancreatic and Hepatic Oncology ResearchLung Cancer Research Studies